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zetasizer dls nano zs system version 7 12  (Malvern Panalytical)
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Characterization of EVs derived from hUC-MSCs. ( A ) SEM provides an image illustrating the geometry and size of the EVs. Scale bar = 5 μm. ( B ) AFM presents a topographic 2D image (amplitude modulation), demonstrating EV adhesion to mica, drying with N2, and captured in air. Scale bar = 1 μm. ( C ) DLS analysis using the Nano <t>Zetasizer</t> reveals the size distribution of EVs. The peak height indicates an average size of 403.3 ± 85.94 nm, while the zeta potential analysis shows a negative potential of approximately −0.270 ± 4.02 mV. A negative zeta potential suggests stability in an aqueous solution, with a value of 4.02 mV indicating stable aqueous dispersion in a physiological environment. ( D ) Light and fluorescent microscopy were utilized to evaluate the distribution of EVs isolated from hUC-MSCs Via the differential centrifugation technique. Scale bar = 50 μm. Overall, A – D provides a comprehensive overview of the physical characteristics, size distribution, surface properties, and molecular composition of the isolated EVs, enhancing our understanding of their potential biological functions and therapeutic applications.
Zetasizer Dls Nano Zs System Version 7 12, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zetasizer dls nano zs system version 7 12/product/Malvern Panalytical
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enterprise guide version 7.12  (SAS institute)
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SAS institute enterprise guide version 7.12
Characterization of EVs derived from hUC-MSCs. ( A ) SEM provides an image illustrating the geometry and size of the EVs. Scale bar = 5 μm. ( B ) AFM presents a topographic 2D image (amplitude modulation), demonstrating EV adhesion to mica, drying with N2, and captured in air. Scale bar = 1 μm. ( C ) DLS analysis using the Nano <t>Zetasizer</t> reveals the size distribution of EVs. The peak height indicates an average size of 403.3 ± 85.94 nm, while the zeta potential analysis shows a negative potential of approximately −0.270 ± 4.02 mV. A negative zeta potential suggests stability in an aqueous solution, with a value of 4.02 mV indicating stable aqueous dispersion in a physiological environment. ( D ) Light and fluorescent microscopy were utilized to evaluate the distribution of EVs isolated from hUC-MSCs Via the differential centrifugation technique. Scale bar = 50 μm. Overall, A – D provides a comprehensive overview of the physical characteristics, size distribution, surface properties, and molecular composition of the isolated EVs, enhancing our understanding of their potential biological functions and therapeutic applications.
Enterprise Guide Version 7.12, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enterprise guide version 7.12/product/SAS institute
Average 90 stars, based on 1 article reviews
enterprise guide version 7.12 - by Bioz Stars, 2026-05
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Characterization of EVs derived from hUC-MSCs. ( A ) SEM provides an image illustrating the geometry and size of the EVs. Scale bar = 5 μm. ( B ) AFM presents a topographic 2D image (amplitude modulation), demonstrating EV adhesion to mica, drying with N2, and captured in air. Scale bar = 1 μm. ( C ) DLS analysis using the Nano Zetasizer reveals the size distribution of EVs. The peak height indicates an average size of 403.3 ± 85.94 nm, while the zeta potential analysis shows a negative potential of approximately −0.270 ± 4.02 mV. A negative zeta potential suggests stability in an aqueous solution, with a value of 4.02 mV indicating stable aqueous dispersion in a physiological environment. ( D ) Light and fluorescent microscopy were utilized to evaluate the distribution of EVs isolated from hUC-MSCs Via the differential centrifugation technique. Scale bar = 50 μm. Overall, A – D provides a comprehensive overview of the physical characteristics, size distribution, surface properties, and molecular composition of the isolated EVs, enhancing our understanding of their potential biological functions and therapeutic applications.

Journal: Biomedicines

Article Title: Extracellular Vesicles Derived from Human Umbilical Cord-Mesenchymal Stem Cells Ameliorate Intervertebral Disc Degeneration

doi: 10.3390/biomedicines13102420

Figure Lengend Snippet: Characterization of EVs derived from hUC-MSCs. ( A ) SEM provides an image illustrating the geometry and size of the EVs. Scale bar = 5 μm. ( B ) AFM presents a topographic 2D image (amplitude modulation), demonstrating EV adhesion to mica, drying with N2, and captured in air. Scale bar = 1 μm. ( C ) DLS analysis using the Nano Zetasizer reveals the size distribution of EVs. The peak height indicates an average size of 403.3 ± 85.94 nm, while the zeta potential analysis shows a negative potential of approximately −0.270 ± 4.02 mV. A negative zeta potential suggests stability in an aqueous solution, with a value of 4.02 mV indicating stable aqueous dispersion in a physiological environment. ( D ) Light and fluorescent microscopy were utilized to evaluate the distribution of EVs isolated from hUC-MSCs Via the differential centrifugation technique. Scale bar = 50 μm. Overall, A – D provides a comprehensive overview of the physical characteristics, size distribution, surface properties, and molecular composition of the isolated EVs, enhancing our understanding of their potential biological functions and therapeutic applications.

Article Snippet: The size distribution and zeta potential of EVs derived from hUC-MSCs were assessed using the Zetasizer DLS Nano ZS system version 7.12 (MAL1160489, Malvern Instruments, Malvern, UK) according to the manufacturer’s guidelines.

Techniques: Derivative Assay, Zeta Potential Analyzer, Dispersion, Microscopy, Isolation, Centrifugation